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STR Genotyping
MLS
BioDNA Ltd performs genotyping of microsatellite markers (STRs) for
genome-wide scans and fine mapping according to the client’s needs.
Microsatellite genotyping has been used in linkage analysis for the
identification of genes responsible for both Mendelian and
non-Mendelian traits.
Genotyping is performed by a group of experienced scientists and by
using the latest technology for DNA fragment analysis.
Requirements and Submission
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We accept existing assays just to run
them for you on the capillary sequencer. These should be
submitted to the laboratory in clearly labelled 96 well
plates, preferably using fluorescent dyes FAM, VIC, NED, PET
and LIZ.
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We also perform genotyping according to
your needs including optimization of PCRs of microsatellites
chosen to suit your chromosomal regions and density,
preferably from the deCode and/or Marshfield’s genetic maps.
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We offer our services for whole genome
scans both for genotyping and analysis of data. Please enquire
for any further details.
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For analysis we accept DNA samples that
are clearly labeled with a unique number. The quantity of DNA
submitted must be twice the amount needed for the whole
genotyping process, where 10ng of DNA is needed for each PCR.
Preferably DNA samples must have a concentration of at least
20ng/ul and should be submitted in TE buffer.
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It is recommended that you check the
quality of DNA by performing PCR and checking PCR products on
agarose. Also please submit the concentrations for each
sample. If DNA concentrations are not given we will measure
DNA concentration by real time PCR (at an extra cost).
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For further information about our
genotyping services and other custom based research projects
please contact us
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Results
After electrophoresis all results will be analysed by one of our
scientists and results will be sent to the client by email and/or CD
Prices in Euros
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Code#
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Academic
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Non-Academic
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GT001
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Electrophoresis per used lane (per
sample)*
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€ 3.50
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€ 4.65
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-
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Electrophoresis per empty lane
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€ 3.00
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€ 4.08
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GT002
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PCR optimization **
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€ 139.79
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€ 151.44
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GT003
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PCR reaction
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€ 1.05
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€ 1.51
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GT004
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Genome-wide
Scan ***
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€ 605.77
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€ 698.97
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*Each run
takes 4 samples
**Price includes ordering of primers and PCR optimization (excluding
electrophoresis)
*** Price per sample including PCR and electrophoresis of 405 STRs
Prices are excluding any VAT and do not include delivery expenses
Single Nucleotide Polymorphism (SNP) Genotyping
SNPs are
DNA sequence variations that occur when a single nucleotide in the
genome is altered (eg: C to T) and that occurs at a frequency of >1%
within a population. The genome is more than 99% identical between
humans coming from different populations. On the other hand SNPs
make up approximately 90% of this very small variation and are
responsible for an individual's susceptibility to disease, response
to treatment (pharmacogenetics) and other traits that make us humans
unique from one another.
A large
number of SNPs have been identified and are found in public
databases. SNP genotyping is useful for the identification of
individuals at risk of disease, response to treatment and for the
localisation of novel genes by linkage disequilibrium (LD) mapping.
MLS BioDNA Ltd is offering a SNP genotyping service using
the TaqMan probe technique. As part of our SNP genotyping service we
design, validate and perform genotyping of your samples at a very
reasonable price.
The price
depends upon the number of SNPs and samples to be analysed. For any
further details and requests please enquire.
You must
first send to us a file with the information about the SNPs you want
to genotype, including the sequence with the marked SNP. At least
the sequence should be about 400bp in length with the marked SNP in
the middle written as shown in this example: ACGTCT[C/T]TCGTC
Using
this information we will be able to design the primers and probes
using appropriate software. If the particular SNP is very difficult
to be analysed by the TaqMan technique we will immediately inform
you. If everything will be ok, we will then order the primers and
probes and start to optimise and validate the method.
We will
then ask you to submit the samples, that should be sent to the
laboratory in tubes or plates, with a unique number for each sample.
Web resources for SNP and gene information
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