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MLS BioDNA
Ltd has all the facilities to participate
and collaborate with research institutes, individual researchers,
Pharmaceutical industry and other research teams in various types of
projects. MLS BioDNA Ltd can also offer DNA sequencing
and genotyping (STRs and SNPs) services to both academic and
industrial researchers, using fluorescent capillary electrophoresis
and real-time PCR technologies.
DNA Sequencing
MLS BioDNA Ltd offers a
sequencing service of plasmids and PCR products for academic,
industrial, and pharmaceutical clients using the Big Dye chemistry
technique and the latest technology in capillary electrophoresis.
For our
standard sequencing service, the template and primers are supplied
by the client. In our laboratories we perform cleaning of products
(upon request), cycle sequencing and capillary electrophoresis. The
data will be sent to the client as requested either by email or on
CD.
Sample submission
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We recommend sending PCR
products in volumes of 25 or 50µL (recommended) and a
concentration of 10 – 50ng/µL. |
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If sending more than 25
samples, please send them in a well sealed 96 well microtitre
plate that is clearly labeled. Also please check the quality and
quantity of PCR products by an agarose gel electrophoresis for
any contaminating fragments prior to submission, also submitting
an image of the gel. |
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Cleaning of PCR products
can be done by you or else by using our cleaning service.
Template should be free of proteins, RNA, genomic DNA, EDTA and
salts. Please use distilled water for elution. |
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Ideal fragment size for
sequencing should be of up to 600 - 650bp. |
Primer requirements
As part of our sequencing service we provide the following standard
sequencing primers:
| M13 uni (-21) |
TGT AAA ACG ACG GCC
AGT |
| M13 rev (-29) |
CAG GAA ACA GCT ATG ACC |
| M13 uni (-43) |
AGG GTT TTC CCA GTC ACG ACG TT |
| M13 rev (-49) |
GAG CGG ATA ACA ATT TCA CAC AGG |
Primers supplied by
customers should meet the following characteristics:
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Primers should be supplied at a concentration of 50pmol/µL, and
at a volume of 30µL in 0.2mL tubes. |
| Ideal primer length
should be between 20 and 30 base pairs with GC content of 40 –
50% |
| Primer’s Tm should
be above 55°C |
| Primers should be
re-suspended in sterile distilled water. |
Results
Results will be submitted to customers either by email or on CD.
Sequencing files can be viewed using a freeware version of Chromas
which can be found using the following link:
http://www.technelysium.com.au/chromas.html
Prices
For our service we offer prices both with and without the cleaning
service, for academics and another for commercial
purposes. Prices also depends on number of samples so please contact
us for further details. We also offer the service to design the
whole experiment starting from primer design, PCR optimisation
followed by sequencing. Prices vary depending on project, so please
contact us with project details and our team of experienced
scientists will guide you and also give you a quotation.
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