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MLS BioDNA Ltd has all the facilities to participate and collaborate with research institutes, individual researchers, Pharmaceutical industry and other research teams in various types of projects. MLS BioDNA Ltd can also offer DNA sequencing and genotyping (STRs and SNPs) services to both academic and industrial researchers, using fluorescent capillary electrophoresis and real-time PCR technologies.
DNA Sequencing
MLS BioDNA Ltd offers a sequencing service of plasmids and PCR products for academic, industrial, and pharmaceutical clients using the Big Dye chemistry technique and the latest technology in capillary electrophoresis.
For our standard sequencing service, the template and primers are supplied by the client. In our laboratories we perform cleaning of products (upon request), cycle sequencing and capillary electrophoresis. The data will be sent to the client as requested either by email or on CD.
Sample submission
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We recommend sending PCR products in volumes of 25 or 50ul (recommended) and a concentration of 10 – 50ng/ul. |
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If sending more than 25 samples, please send them in a well sealed 96 well microtitre plate that is clearly labeled. Also please check the quality and quantity of PCR products by an agarose gel electrophoresis for any contaminating fragments prior to submission, also submitting an image of the gel. |
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Cleaning of PCR products can be done by you or else by using our cleaning service. Template should be free of proteins, RNA, genomic DNA, EDTA and salts. Please use distilled water for elution. |
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Ideal fragment size for sequencing should be of up to 600 - 650bp. |
Primer requirements
As part of our sequencing service we provide the following standard sequencing primers:
| M13 uni (-21) |
TGT AAA ACG ACG GCC AGT |
| M13 rev (-29) |
CAG GAA ACA GCT ATG ACC |
| M13 uni (-43) |
AGG GTT TTC CCA GTC ACG ACG TT |
| M13 rev (-49) |
GAG CGG ATA ACA ATT TCA CAC AGG |
Primers supplied by customers should meet the following characteristics:
| Primers should be supplied at a concentration of 50pmol/µl, and at a volume of 30µl in 0.2ml tubes. |
| Ideal primer length should be between 20 and 30 base pairs with GC content of 40 – 50% |
| Primer’s Tm should be above 55ºC |
| Primers should be re-suspended in sterile distilled water. |
Results
Results will be submitted to customers either by email or on CD. Sequencing files can be viewed using a freeware version of Chromas which can be found using the following link: http://www.technelysium.com.au/chromas.html
Prices
For our service we offer prices both with and without the cleaning service, for academics and another for commercial purposes. Prices also depends on number of samples so please contact us for further details. We also offer the service to design the whole experiment starting from primer design, PCR optimisation followed by sequencing. Prices vary depending on project, so please contact us with project details and our team of experienced scientists will guide you and also give you a quotation.
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